morphoМорфологические ведомостиMorphological newsletter1812-31712686-8741Private Medical University REAVIZ10.20340/mv-mn.18(26).02.32-37morpho-117Research ArticleМОРФОМИКА - НОВЫЕ ТЕХНОЛОГИИPART 3 - MORPHOMICS - NEW TECHNOLOGIESВЛИЯНИЕ НАНОЧАСТИЦ ДИОКСИДА ТИТАНА НА СТРУКТУРНЫЕ ОСОБЕННОСТИ ГИППОКАМПА КРЫСTHE INFLUENCE OF THE TITANIUM DIOXIDE NANOPARTICLES ON STRUCTURAL FEATURES OF THE RATS HIPPOCAMPUSШарафутдиноваЛ. А.SharafutdinovaL. A.sharafla@yandex.ruВалиуллинВ. В.ValiullinV. V.valvv@kcn.ruБашкирский государственный университетРоссияBashkir State UniversityRussian FederationКазанский государственный медицинский университетРоссияKazan State Medical UniversityRussian Federation2018300620182623237Copyright © Шарафутдинова Л.А., Валиуллин В.В., 20182018Шарафутдинова Л.А., Валиуллин В.В.Sharafutdinova L.A., Valiullin V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.morpholetter.com/jour/article/view/117

В связи с широким производством и использованием наночастиц диоксида титана (НЧ TiO2), обладающих токсическими эффектами, возрастает риск их неблагоприятного воздействия на функции различных систем организма человека и, в первую очередь, нервной, ввиду ее чрезвычайно высокой чувствительности к любым повреждающим агентам. Цель работы: изучение структурных характеристик, ультрамикроскопических особенностей СА1 и СА3 областей гиппокампа крыс и уровня экспрессии маркера астроцитов - кислого глиального фибриллярного белка (GFAP) в условиях интраназального введения НЧ TiO2. Использованы гистологические, иммуногистохимические и электронно-микроскопические методы исследования. Установлено, что на фоне интраназального введения НЧ TiO2 в полях СА1 и СА3 гиппокампа развиваются дегенеративные изменения нейронов и астроглиоз. Электронно-микроскопическое исследование выявило признаки повреждения энергетического и белоксинтетического аппаратов нейронов. Результаты проведенного исследования позволили сделать вывод о том, что НЧ TiO2 оказывают нейротоксический эффект на структурные характеристики гиппокампа крыс при интраназальном введении.

Due to the wide production and use of titanium dioxide nanoparticles (NP TiO2), which have toxic effects, the risk of their adverse effect on the functions of human bodys various systems and, primarily, nervous, increases because of its extremely high sensitivity to any damaging agents. Objective: to study the structural characteristics, ultramicroscopic features of the rat hippocampus CA1 and CA3 regions, and the level of the astrocyte marker - acid glial fibrillar proteins (GFAP) expression under intranasal administration of NP TiO2. Histological, immunohistochemical and electron-microscopic methods were used. It was established that against the background of intranasal administration of NP TiO2 in the CA1 and CA3 regions of the hippocampus, degenerative changes in neurons and astrogliosis develop. Electron-microscopic examination revealed signs of damage to the energy and protein-synthesizing apparatus of neurons. The results of the study made it possible to conclude that NP TiO2 have a neurotoxic effect on the structural characteristics of the rat hippocampus in intranasal administration.

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The authors declare that there are no conflicts of interest present.