morphoМорфологические ведомостиMorphological newsletter1812-31712686-8741Private Medical University REAVIZ10.20340/mv-mn.2016.24(4):81-85morpho-221Research ArticleКРАТКИЕ СООБЩЕНИЯSHORT ARTICLESФИБРОНЕКТИН ЩИТОВИДНОЙ ЖЕЛЕЗЫ СОБАК ПРИ ОДНОКРАТНЫХ И МНОГОКРАТНЫХ МЫШЕЧНЫХ НАГРУЗКАХFibronectin of the thyroid gland in dogs at single and multiple muscle loadsБезденежныхАндрей ВячеславовичBezdenezhnykhA. V.natom311@gmail.comФГБОУ ВО «Нижегородская государственная медицинская академия» Минздрава РоссииRussian Federation2016301220162448185Copyright © Безденежных А.В., 20162016Безденежных А.В.Bezdenezhnykh A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.morpholetter.com/jour/article/view/221

Известна роль фибронектина (ФН) в дифференцировке, росте, поддержании нормальной морфологии, пролиферации и апоптозе клеток, обеспечении их миграции и пространственной организации, последнее осуществляется за счет ремоделирования сосудистого и нервного обеспечения. Цель исследования - изучить ФН щитовидной железы (ЩЖ) в ответ на различные видь мышечной деятельности. Работа выполнена на ЩЖ собак-самцов контрольной группы (16) и экспериментальных групп (67), которые имели различные режимы бега: кратковременные - несколько минут и длительные - несколько месяцев. Для выделения ФН применяли моноклональные антитела. После качественного анализа проводили оцифровку срезов правой доли органа и измеряли площадь фибронектина. Исследования выявили, что однократные тренирующие и многократные нагрузки длительностью от одного до трех месяцев не оказывали существенного влияния на ФН ЩЖ. При однократных нагрузках стартового характера с минимальным временем бега и предельных нагрузках с максимальным временем бега площади ФН были больше, чем в контрольной группе и достоверно отличались друг от друга. B этих группах 4CC увеличивалась, выявлены гиперемия сосудистого русла ЩЖ и признаки гиперфункции органа. При стартовых нагрузках объем ФН повышался за счет накопления интраваскулярной фракции, тогда как при предельных нагрузках его внутри- и межфолликулярное накопление, возможно, обусловлено активизацией тироцитов. Таким образом, фибронектин, который традиционно рассматривается как маркер процессов новообразований щитовидной железы, может служить отражением пролиферативной активности тироцитов при мышечных нагрузках.

Fibroneetin (FN) plays a role in the differentiation, growth, maintenance of normal morphology, proliferation and apoptosis of cells, their migration and spatial organization, ineluding due to remodeling of the vascular and nervous supply. The aim of the study is FN in the thyroid gland (TG) after various types of muscular activity. The control thyroid-intact male dogs (16) have no running. Six experimental groups include 67 dogs with different models of running (from a few minutes to a few months). Monoclonal antibodies were used to isolate FN. Sections of the right lobe of TG were digitizing and areas were measured after qualitative analysis. Areas of FN after single loads with middle running time and multiple loads (from one to three months) did not differ significantly from the control. Areas of FN in two groups (single loads with minimum running time and single loads with maximum running time) were more than in the control group and they are significantly different from each other. The heart rate and vascular supply of TG increased; morphologie signs of thyroid proliferation were present at dogs of these groups. Accumulation of intravascular faction of FN was recognized at the single loads with minimum running time. Accumulation of interfollicular and intrafollieular factions of FN was found at the single loads with maximum running time, it is possible due to the activation of thyroeytes. Thus, fibronectin, which is traditionally regarded as a marker of tumors of the thyroid gland, can serve as a reflection of proliferative activity of thyrocytes after the muscle loads.

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The authors declare that there are no conflicts of interest present.