morphoМорфологические ведомостиMorphological newsletter1812-31712686-8741Private Medical University REAVIZ10.20340/mv-mn.2023.31(1).758morpho-758Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯRESEARCH ARTICLESВЛИЯНИЕ ПОСТОЯННОГО ОСВЕЩЕНИЯ НА УЛЬТРАСТРУКТУРУ ГЕПАТОЦИТОВ КРЫСTHE INFLUENCE OF THE CONSTANT ILLUMINATION ON THE ULTRASTRUCTURE OF RAT'S HEPATOCYTEShttps://orcid.org/0000-0001-6251-2560АрешидзеДавид АлександровичAreshidzeDavid A.

кандидат биологических наук, заведующий лабораторией патологии клетки Научно-исследовательского института морфологии человека имени академика А.П. Авцына

Candidate of Biological Sciences, Head of the Laboratory of Cell Pathology of the Academician Avtsyn Research Institute of Human Morphology

labcelpat@mail.ruhttps://orcid.org/0000-0001-6251-2560КозловаМария АлександровнаKozlovaMariya A.

научный сотрудник лаборатории патологии клетки Научно-исследовательского института морфологии человека имени академика А.П. Авцына

Scientific Researcher of the Laboratory of Cell Pathology of the Academician Avtsyn Research Institute of Human Morphology

ma.kozlova2021@outlook.comhttps://orcid.org/0000-0002-3253-6729ЧерниковВалерий ПетровичChernikovValery P.

кандидат медицинских наук, ведущий научный сотрудник лаборатории патологии клетки Научно-исследовательского института морфологии человека имени академика А.П. Авцына

Candidate of Medical Sciences, Leading Scientific Researcher of the Laboratory of Cell Pathology of the Academician Avtsyn Research Institute of Human Morphology

1200555@mail.ruhttps://orcid.org/0000-0002-8096-5974КондашевскаяМарина ВладиславовнаKondashevskayaMarina V.

доктор биологических наук, доцент, главный научный сотрудник лаборатории патологии клетки Научно-исследовательского института морфологии человека имени академика А.П. Авцына

Doctor of Biological Sciences, Docent, Main Scientific Researcher of the Laboratory of Cell Pathology of the Academician Avtsyn Research Institute of Human Morphology

nihilist78@mail.ruРоссийский научный центр хирургии имени академика Б.В. Петровского, МоскваРоссияAcademician Petrovsky Scientific Centre of Surgery, MoscowRussian FederationРоссийский научный центр хирургии имени академика Б.В. Петровского, Москва .РоссияAcademician Petrovsky Scientific Centre of Surgery, MoscowRussian Federation2023050120233114653Copyright © Арешидзе Д.А., Козлова М.А., Черников В.П., Кондашевская М.В., 20222022Арешидзе Д.А., Козлова М.А., Черников В.П., Кондашевская М.В.Areshidze D.A., Kozlova M.A., Chernikov V.P., Kondashevskaya M.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.morpholetter.com/jour/article/view/758

Дезорганизация природных биоритмов в современном мире чаще всего связана с нарушением циркадианных ритмов из-за светового загрязнения. Урбанизация напрямую взаимосвязана с избытком искусственного освещения. Как разовое явление световое загрязнение приводит к обратимому сдвигу циркадианных ритмов, а при постоянном воздействии - к развитию десинхроноза. Известно, что световое загрязнение способствует развитию неалкогольной жировой дистрофии печени, первичного билиарного цирроза, нарушению обмена веществ. Целью исследования было изучение изменений микроморфометрических показателей и ультраструктуры гепатоцитов крыс линии Вистар под влиянием обычного освещения и постоянного светового воздействия. Исследование проведено на 120 беспородных крысах-самцах линии Вистар в возрасте 6 месяцев, массой тела 350 грамм. Крыс разделили на 2 равные группы. В контрольную группу вошли 60 крыс, содержавшихся в стандартных лабораторных условиях при обычном циклическом «свет-темнота» световом режиме (10:14, 10 часов света — с 8:00 до 18:00, 14 часов темноты — с 18:00) в течение 3 недель. В экспериментальную группу вошли 60 крыс, которых содержали в стандартных лабораторных условиях при постоянном освещении также в течение 3 недель. Для получения результатов использовались гистологические, микроморфометрические методы и просвечивающая электронная микроскопия. Выявленные изменения гепатоцитов под влиянием постоянного освещения свидетельствуют о том, что нарушение режима освещения является сильнодействующим фактором, вызывающим повреждение и структурные изменения клеток печени. Понимание механизмов, лежащих в основе реакции клеток печени на нарушение циркадного ритма и связанное с ним повреждение, важно для формирования индивидуальных рекомендаций по образу жизни и режимам поведения.

The disorganization of natural biorhythms in the modern world is mostly attributed to the violation of circadian rhythms due to light pollution. Urbanization is directly interconnected with an excess of artificial lighting. As a one-time phenomenon, light pollution leads to a reversible shift in circadian rhythms, but in the case of constant influence, it leads to the development of desynchronosis. It is known that light pollution contributes to the development of nonalcoholic fatty liver disease, primary biliary cirrhosis, and metabolic disorders. The study aimed to investigate the changes in micro-morphometric parameters and ultrastructure of hepatocytes of Wistar rats under the influence of normal lighting and constant lighting exposure. This study was conducted on 120 outbred stock male Wistar rats at an age of 6 months, with a body weight of 350 g. The rats were divided into 2 equal groups. The control group included 60 rats, kept in standard laboratory conditions under a normal cyclical «light-dark» lighting regime (10:14, 10 hours of light – from 8:00 to 18:00, 14 hours of darkness – from 18:00 to 8:00) within 3 weeks. The experimental group included 60 rats kept in standard laboratory conditions under constant lighting within 3 weeks. To obtain the results, histological, micro morphometric methods and transmission electronic microscopy were used. The revealed changes of the hepatocytes under the influence of constant lighting indicate that a violation of the illumination regime is a potent factor causing damage and structural changes in the liver. Understanding the mechanisms underlying the liver's response to circadian rhythm disruption and associated damage is important to form patient-specific recommendations on lifestyle and behavioral regimens.

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The authors declare that there are no conflicts of interest present.