morphoМорфологические ведомостиMorphological newsletter1812-31712686-8741Private Medical University REAVIZ10.20340/mv-mn.2024.32(2).838morpho-838Research ArticleКРАТКИЕ СООБЩЕНИЯSHORT ARTICLESАДГЕЗИВНЫЕ СВОЙСТВА МАКРОФАГОВ ПОТОМСТВА САМОК КРЫС ПРИ ХРОНИЧЕСКОМ ВОЗДЕЙСТВИИ БИСФЕНОЛА АFEMALE RAT'S OFFSPRING MACROPHAGES' ADHESIVE PROPERTIES AT BISPHENOL A CHRONIC EXPOSUREhttps://orcid.org/0009-0003-2892-6390ГуминаАнна АндреевнаGuminaAnna A.

ассистент кафедры гистологии, эмбриологии и цитологии

Assistant of the Histology, Embryology and Cytology Department

annagumina3@gmail.comhttps://orcid.org/0009-0006-4456-9372МосквинаАнастасия ОлеговнаMoskvinaAnastasiya O.

старший лаборант кафедры гистологии, эмбриологии и цитологии, преподаватель медицинского колледжа

Senior Laboratorin of the Histology, Embryology and Cytology Department, Lecturer of the Medical College

moskvina.n.98@mail.ruЮжно-Уральский государственный медицинский университет, ЧелябинскРоссияSouth Ural State Medical University, ChelyabinskRussian Federation202402092024322ID-838 статья опубликована - Article is publishedCopyright © Гумина А.А., Москвина А.О., 20242024Гумина А.А., Москвина А.О.Gumina A.A., Moskvina A.O.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.morpholetter.com/jour/article/view/838

Бисфенол А является одним из самых часто встречающихся эндокринных дизрапторов, входящих в состав поликарбонатных пластиков, из которых изготавливается широкий спектр изделий для ежедневного использования (пищевые тары, детские игрушки, водосточные трубы, стоматологические материалы и другие). Известно, что иммунорезистентность организма во многом определяется активностью клеточных элементов системы мононуклеарных фагоцитов. В связи с вышеуказанным, целью исследования явился анализ влияния бисфенола А на способность к адгезии и распластыванию макрофагов легкого, брюшной полости и моноцитов периферической крови. Исследование выполнено на половозрелых белых лабораторных самках крыс линии Wistar, которые в течение 45 дней ежедневно подвергались воздействию бисфенола А путем принудительного введения раствора под язык в дозе 15000 мкг/кг. Объектом исследования явилось их 60-ти дневное потомство на стадии ранней половой зрелости. Оценивалось содержание макрофагов, их способность к адгезии и распластыванию при разном времени инкубации. Под влиянием бисфенола А происходит угнетение адгезивных свойств мононуклеарных фагоцитов, о чем свидетельствует уменьшение числа адгезировавшихся клеток, в том числе моноцитов периферической крови на 28,7%, перитонеальных макрофгов на 34,6%, а альвеолярных макрофагов на 35,2. Также содержание адгезированных клеток при исследовании перитонеальных макрофагов, альвеолярных макофагов и моноцитов периферической крови снижалось в опыте на 24,7%, 27,2% и 50,5%, соответственно. Бисфенол А через плацентарный кровоток проникает в кровь плода и оказывает негативное влияние на процесс гемопоэза, в том числе моноцитопоэз, что в конечном итоге обусловливает нарушение становления рецепторного аппарата тканевых и циркулирующих макрофагов. Таким образом, хроническое воздействие бисфенола А на организм самок крыс обусловливает изменение содержания макрофагов различных популяций у потомства, а также угнетает их способность к адгезии и распластыванию, что свидетельствовует о несостоятельности рецепторного аппарата клеток.

Bisphenol A is one of the most common endocrine disruptors found in polycarbonate plastics used to manufacture a wide range of everyday products (food containers, children's toys, drainpipes, dental materials, etc.). It is known that the body's immune-resistance is largely determined by the activity of cellular elements of the mononuclear phagocyte system. In connection with the above, the aim of the study was to analyze the effect of bisphenol A on the ability of lung and abdominal macrophages and peripheral blood monocytes to adhere and spread. The study was performed on mature white laboratory female Wistar rats, which were exposed to bisphenol A daily for 45 days by forced sublingual treatment of the solution at a dosage of 15,000 μg/kg. The object of the study was their 60-day-old offspring at the stage of early sexual maturity. The content of macrophages, their ability to adhere and spread at different incubation times were assessed. Under the influence of bisphenol, A, the adhesive properties of mononuclear phagocytes are inhibited, as evidenced by a decrease in the number of adhered cells, including peripheral blood monocytes by 28.7%, peritoneal macrophages by 34.6%, and alveolar macrophages by 35.2. Also, the content of adhered cells in the study of peritoneal macrophages, alveolar macrophages and peripheral blood monocytes decreased in the experiment by 24.7%, 27.2% and 50.5%, respectively. Bisphenol A penetrates into the fetal blood through the placental bloodstream and has a negative effect on the process of hematopoiesis, including monocyte development, which ultimately causes a disruption in the formation of the receptor apparatus of tissue and circulating macrophages. Thus, chronic exposure of female rats to bisphenol A causes a change in the content of macrophages in various compartments of the offspring, and also inhibits their ability to adhere and spread, which indicates the failure of the receptor apparatus of cells.

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The authors declare that there are no conflicts of interest present.