CHARACTERIZATION OF INTERCELLULAR RELATIONSHIPS IN HIV/TB CO-INFECTION TAKING INTO ACCOUNT THE EXPRESSION OF BIOMARKERS CD3⁺, CD7⁺, CD10⁺ IN THE LUNGS

The study of morphological changes directly in areas of inflammatory response in HIV/TB coinfection allow to estimate the functional activity and intercellular relationships of immune cells. The purpose of the study was to assess the degree of expression of CD3⁺,CD7⁺,CD10⁺ biomarkers in foci of granulomatous inflammation in lung tissues of patients who died of HIV / TB co-infection. Autopsy material (n=30) of patients with HIV/TB co-infection was studied, stage 4B-5 - group 1. In the comparison group - a group 2 - includes autopsy cases (n=30) with mono-infection with TB. Immunohistochemical investigation to determine the expression of biomarkers of CD3⁺,CD7⁺,CD10⁺ was performed with using rabbit monoclonal antibodies (Epitomics, Abcam). The severity of biomarker expression was assessed with the use of Image Analysis with subsequent morphometric analysis. The author showed a reduction in the number of morphologically mature CD3+ T-lymphocytes and an increase in the number of immature CD7⁺ T-lymphocytes mainly in foci of productive inflammation, indicating a profound inhibition of the immune response in HIV infection in the terminal stages of immunodeficiency, with the development of tuberculosis. The decrease in the number of CD10+ cells in foci of productive inflammation in lungs, indicating disruption of the processes of B-lymphocyte differentiation, contributing to a deep depression of humoral immunity with weak immune response to Mycobacterium tuberculosis.

ВИЧ, туберкулез, иммуногистохимия, CD3⁺, CD7⁺, CD10⁺, HIV, tuberculosis, immunohistochemistry, CD3⁺, CD7⁺, CD10⁺

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