THE INFLUENCE OF ACUTE CEREBRAL ISCHEMIA ON THE RATS VENTRICULAR VASCULAR PLEXUS STRUCTURE

Many modern chronic diseases lead to the development of cerebral hypoperfusion (brain ischemia). One of the top problems in the development of cerebral ischemia is the state of its non-neural structural components. The purpose of the study is the quantitative indicators of the structural components of the choroid plexuses of the rat brain at acute artificial ischemia. The object of the study were 30 white Wistar rats weighing 200-220 grams at the age of 4 months, divided into 6 equal groups of 5 animals: control group, experimental groups - after 1 hour, 1 day, 3 days, 7 days, 14 days of the experiment. Anesthetized animals were fixed, both common carotid arteries were prepared, which were then tightly ligated. The wound was treated with an antiseptic and sutured in layers. Then, after 1 hour, on days 1, 3, 7, 14, the rats were sequentially removed from the experiment. To assess the volume of the brain ventricles, sections were selected in which their cross-sectional area was maximum. The volume of the ventricles of the brain was determined on digitized micrographs at a 4-fold magnification by tracing their boundaries and subsequent calculations in the morphometric computer program ImageJ. Determination of volumetric fractions of the choroid plexuses was carried out by the stereological method on 50 serial sections using an ocular grid with 60 equidistant intersection nodes on a Micromed microscope at a magnification of 400 times. As a result of the study, it was established that during acute experimental cerebral ischemia, the choroid plexuses of the ventricles undergo significant structural changes at different time intervals, and the general and vascular architecture changes in all parts of the ventricles. In the lateral ventricles, the maximum area increases, the relative volume of the choroid plexuses decreases, the volume fraction of vessels inside the plexus increases, and the volume fraction of cells decreases. Other ventricles are characterized by specific dynamics. The results obtained contribute to the mechanisms of the pathogenesis of acute cerebral ischemia and its consequences.

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