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THE STUDY OF THE RATIO OF SUBPOPULATIONS OF LYMPHOCYTES IN THE CAPILLARY BLOOD OF THE PAPULE AND IN THE VENOUS BLOOD IN PATIENTS WITH THE PSORIASIS

https://doi.org/10.20340/mv-mn.2018.26(3):31-36

Abstract

Understanding the migration processes of various morphological populations of lymphocytes, as well as the conditions under which this migration can be activated or slowed down, is especially important for interpreting the stability reserves and maintaining homeostasis. The purpose of the study is to comparison of the ratio of the subpopulations of capillary blood lymphocytes in psoriatic papules and in venous blood, depending on their morphology and receptors expression in patients with psoriasis. A clinical and immunological examination of 82 patients aged from 20 to 60 years, suffering from vulgar and exudative psoriasis in progressive and inpatient stages was carried out. Disease duration ranged from 3 months to 10 years. As a control group, 50 practically healthy individuals aged from 20 to 60 years who had no acute chronic diseases or their exacerbations at the time of the survey were examined. On smears of venous blood, and blood obtained from the surface of psoriatic papules (the phenomenon of point bleeding during the psoriatic triad), fixed in a mixture of Nikiforov and stained by Romanowsky-Giemsa, determined the concentration of lymphocytes among other leukocytes. Lymphocytes was differentiated according to the size of the cell, taking into account the size of the cytoplasm: small lymphocytes - up to 8 microns, medium - from 8 to 12 microns, large - more than 12 microns. Venous blood for the study was taken in the morning on an empty stomach. By using the flow cytometer FC-500 Beckman Coulter (USA) was determined the content of L-selectin (CD62L, FITC), LFA-1 (CD11a, FITC), LFA-3 (CD58, FITC), ICAM-1 (CD54, FITC), PECAM -1 (CD31, FITC) on whole blood mononuclears. The results show that the greatest changes were related to the content in the venous blood of large lymphocytes, the level of which was significantly reduced, whereas in psoriatic papule, on the contrary, it was sharply increased. Large lymphocytes are known to be evidence of lymphoproliferation. The inflammatory process is accompanied by lymphoproliferation. On the other hand, statistically significant correlations between concentrations of large lymphocytes and lymphocytes expressing receptors for LFA-1, ICAM-1, LFA-3 and PECAM-1 and the absence of such correlations in the comparison group, may additionally indicate the preferential migration of lymphocytes that have entered lymphoproliferation.

About the Authors

A. K. Sherstennikova
Северный государственный медицинский университет
Russian Federation


S. L. Kashutin
Северный государственный медицинский университет
Russian Federation


V. S. Neklyudova
Северный государственный медицинский университет
Russian Federation


O. V. Kalmin
Пензенский государственный университет
Russian Federation


V. I. Nikolaev
Северо-Западный государственный медицинский университет им. И.И. Мечникова
Russian Federation


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For citations:


Sherstennikova A.K., Kashutin S.L., Neklyudova V.S., Kalmin O.V., Nikolaev V.I. THE STUDY OF THE RATIO OF SUBPOPULATIONS OF LYMPHOCYTES IN THE CAPILLARY BLOOD OF THE PAPULE AND IN THE VENOUS BLOOD IN PATIENTS WITH THE PSORIASIS. Morphological newsletter. 2018;26(3):31-36. (In Russ.) https://doi.org/10.20340/mv-mn.2018.26(3):31-36

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