THE DISTRIBUTION OF MOLECULES OF THE INTERCELLULAR ADHESION ON BLOOD LEUKOCYTES DEPENDING ON THEIR MORPHOLOGY

Physiological understanding of the processes of migration of immunocompetent cells, as well as the conditions under which migration can be activated or slowed down, is especially important for the interpretation of the reserves of stability and preservation of homeostasis. Migration of leukocytes through the endothelium of the microcirculation is regulated by adhesion molecules. The aim of the study was to study the distribution of intercellular adhesion molecules on leukocytes depending on their morphology. An examination of 50 healthy individuals aged 20 to 60 years with no history of chronic disease was carried out. Venous blood for the study was taken in the morning on «empty» stomach. On a flow cytometer FC-500 company Beckman Coulter (USA) determined the number of neutrophils, monocytes and lymphocytes of venous blood containing molecule L-selectin (CD62L), LFA-1 (CD11a), LFA-3 (CD58), ICAM-1 (CD54), PECAM-1 (CD31), while differentiated them based on morphology. The results is shown that more than 50% of neutrophils and 42% of monocytes are ready to enter the slip phase, while the number of lymphocytes entering this phase is relatively small - 11%. In the phase of strong adhesion and transmigration more than 90% of neutrophils and monocytes were ready to enter, while the number of lymphocytes in blood did not exceed 13%. Correlation analysis showed that in the absence of antigenic stimulation the greatest readiness for the implementation of the slip phase, strong adhesion and the transmigration was observed in neutrophils with 5-segments in the nucleus and polymorphonuclear monocytes. Willingness to transmigrate lymphocytes was relatively small and concerned mainly of medium size lymphocytes and large granular lymphocytes.

клетки крови, лейкоциты, молекулы адгезии, L-селектин, blood cells, leukocytes, adhesion molecules, L-selectin