THE RESULTS OF A PATOMORPHOLOGICAL ANALYSIS OF THE BLADDER WALL AT THE INTERSTITIAL CYSTITIS OR PAINFUL BLADDER SYNDROME IN AN EXPERIMENT

The etiology and pathogenesis of painful bladder syndrome or interstitial cystitis is not fully understood. For a better understanding of the pathogenesis of the disease, experimental animal models have been created. It is assumed that the histopathological features of the inflammatory response of the bladder wall may play an important role in predictive modeling of the symptoms of interstitial cystitis. It was found that in samples of bladder preparations with interstitial cystitis, an increase in the number of functionally active mast cells, both partially and completely degranulated, is observed. However, there is no convincing evidence that mast cells can serve as cell markers for this disease. The aim of the investigation was to identify cells of inflammation in histological preparations of the urinary bladder mucosa in 3 experimental models of interstitial cystitis. The study was carried out on 37 female rabbits. Experimental animals were injected into the bladder cavity with protamine sulfate (1^st group); into the wall of the bladder urine taken from the bladder (2^nd group); into the wall of the bladder 0.9% NaCl solution (3rd group). The 4th group of animals was intact. 14 days after the injections, the animals were taken out of the experiment, cystectomy was performed, preparations of the bladder wall and histological sections were prepared. The average number of all types of leukocytes in the tissues of the urinary bladder wall of animals of the 1st group exceeded that in the remaining groups (p <0.001). Mast cells were detected in the preparations of the 1st and 2nd groups, in the 2nd group their number was 25 times higher than in the 1st group (p <0.001). In animals of the 1st group, inflammatory infiltration of the bladder wall with lymphocytes and neutrophils was noted. In 2nd group, infiltration of the bladder wall with inflammatory cells was associated with a large number of mast cells. In the preparations of animals of the 3rd group, edema, single lymphocytes and neutrophils were detected. The data obtained make a certain contribution to the study and understanding of the mechanisms of inflammatory processes in interstitial cystitis.

urinary bladder, inflammation, interstitial cystitis, painful bladder syndrome, experimental models, mast cells

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