Topographical features state of erythrocyte membranes blood experimental vaccines process

Abstract

The development of highly effective and safe vaccines against plague and tularemia increases the demands on the accuracy and informativeness of safety assessment methods developed drugs. The purpose of research - to characterize the surface architecture of erythrocyte membranes (PAME) in the blood of mice immunized with vaccine strains cultures Francisella tularensis 15 lines NIIEG and Yersinia pestis EV line NIIEG at different times. The immunized animals were also registered a decrease in the number of discocytes increasing the number of transformed forms of cells. The severity of the changes PIFFA on the first day of the experiment were in direct relation to the immunizing dose of the vaccine strains of the plague and tularemia microbe. In the process of immunogenesis changes PIFFA was planned reduction in the number of transformed cells in the period from 3 to 21 days of observation. On the histogram curve of the cross section of the cells in all immunized animals were observed deeper wrinkling of the membrane surface, expressed in the early stages of immunogenesis and subsequent smoothing of the contours of erythrocytes that matches the picture of a partial restoration of damaged membrane structure and cell metabolism. Thus, in normal hematopoietic system reacts to external influence actively and quickly restore equilibrium within himself, that allows us to consider the possibility of taking into account the state of PAME to characterize the adaptive-compensatory reactions of microorganism with the vaccine process.