Morphological features of the lymphoid tissue and microvessels in regional lymph nodes in gastric cancer

The morphological features of lymphoid tissue and microvessels in samples of regional lymph nodes without metastases were studied in 44 patients with gastric cancer. The samples were stained with hematoxylin-eosin and immunohistochemically using antibodies to CD34. In lymph nodes identified the following types of microvessels: the usual microvessels, the dilated capillaries and the atypical capillaries that were formed by the cells with the phenotype of the blast. Presence of abnormal capillary endothelial cells of blast phenotype was associated with the degree of differentiation of gastric cancer, the type of tumor growth and the amount of lymph follicles to the border of the tumor and gastric mucosa. Also, positive correlation with the area of the square of germinal centers of lymph follicles, an area of germinal centers, the size of the of lymph follicles, with the number of lymph follicles on the border of the gastric mucosa and tumors and the presence of dilated capillaries in the gastric submucosa and negative - a density of lymph follicles and microvessel density in them. LF area was positively correlated with the area of germinal centers, microvessel density in the germinal centers and negatively - with a density of lymph follicles. Size of lymph follicles were positively correlated with T stage gastric cancer and negatively with the 3-year disease-free survival. Density of lymph follicles was associated with tumor size, N stage and T stage of gastric cancer. Thus, these data suggest that reactive changes of in regional lymph nodes lymphoid tissue and processes angiogenesis in regional lymph nodes associated with inflammatory changes in the stroma of tumors, but also with clinical characteristics and prognosis of gastric cancer.

рак желудка, ангиогенез, лимфатические узлы, лимфоидные фолликулы, Gastric cancer, angiogenesis, lymph nodes, lymphoid follicles

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