New immunomorhpological diagnostic criteria of ileitis in patients with inflammatory bowel disease

Ileal biopsies of 50 patients, from them 18 with Crohn's disease, 11 - with inflammatory bowel disease unclassified and 21 - with not changed mucous membrane are studied. Immunohistochemical research with antibodies to Chromogranin A and CD3 is conducted. At Crohn's disease the number of neuroendocrine cells in one villus was 0 - 28 (mean - 3±8), at inflammatory bowel disease unclassified - 3 - 31 (mean - 15±8), in not changed mucous membrane - 1 - 19 (mean - 9±5). At calculation of quantity of neuroendocrine cells on all crypt it is revealed that at Crohn's disease the minimum and maximum values of the maintenance of these cells were 4 - 24 (mean - 13±5), at inflammatory bowel disease unclassified - 0 - 26 (mean - 14±8), in not changed mucous membrane - 1 - 26 (mean - 9±5). Number CD3+intraepitelial lymphocytes in one villus with the sizes 400 μm and less at Crohn's disease was 7 - 62 (mean - 23±11), at inflammatory bowel disease unclassified - 11 - 49 (mean - 24±8), in not changed mucous membrane - 3 - 50 (mean - 18±9). At calculation of quantity the intraepitelial lymphocytes on 100 and more epithelial cells at the sizes of villus from 401 μm and more, it is revealed that at Crohn's disease the quantity of CD3⁺ cells was 28 - 63 (mean -39,5±11), at inflammatory bowel disease unclassified - 26 - 70 (mean - 46±13), in not changed mucous membrane - 22 - 40 (mean - 29±6). It is established that the average content of neuroendocrine cells and the intraepitelial lymphocytes in the ileal biopsies reliable is above at inflammatory bowel diseases in comparison with not changed mucous membrane that shows their participation in pathogenesis of this group of diseases.

воспалительные заболевания кишечника, болезнь Крона, неклассифицируемый колит, нейроэндокринные клетки, интраэпителиальные лимфоциты, inflammatory bowel diseases, Crohn's disease, inflammatory bowel disease unclassified, neuroendocrine cells, intraepithelial lymphocytes

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