MORPHOLOGICAL FEATURES OF THYROID FOLLICLES AND THE CONSIST OF INTRATHYROID LYMPHOCYTES IN GRAVES' DISEASE
Margarita A. Dudina,
Nadezhda N. Medvedeva,
Andrey A. Savchenko,
Anton V. Moshev,
Evgeny L. Zhukov,
Elena A. Hapilina,
Gul'mira K. Kovalyova,
Sergey A. Dogadin,
Vladimir A. Man'kovsky Abstract
The accumulation of T lymphocytes in the thyroid parenchyma in autoimmune hyperthyroidism is strongly restricted and may indicate the primary role of T cells in the development of Graves' disease (GD). However, the mechanisms of lymphocytes invasive activity in thyroid gland, as well as morphological rearrangement of the thyroid parenchyma in GD are still unknown. The aim of the present study was to establish the morphological features of thyroid follicles and the intrathyroidal lymphocyte subsets in GD. The results of the conducted studies showed that the outcome of tissue-specific autoimmunity and long term antithyroid therapy in GD patients is a rearrangement of the thyroid epithelium with histopathologic signs of Hashimoto's thyroiditis, especially in case of the disease reccurence. In group of GD disease relapse patients the percentage of CD3+CD4+CD127LowCD25High and B1 memory cells were increased (p<0,001), with a decrease in the content of T-activated and cytotoxic activated T-cells, as well as the population of B1 lymphocytes (p<0,01), in comparison with the level estimated in patients with the newly diagnosed disease. In conclusion, our results indicate that intrathyroidal T cell aggression in phase of autoimmune hyperthyroidism, followed by antithyroid therapy, intrathyroidal accumulation of antithyroid drugs with restotaration of the euthyroid state could be the cause of morphofunctional reactions of the thyroid tissue that can make a significant contribution to the immunopathogenesis of the disease.
About the Authors
Margarita A. Dudina
Professor Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk Regional Clinical Hospital, Krasnoyarsk
Russian Federation
Competing Interests:
Russian Federation
Candidate of Medical Sciences, Associate Professor of the Department of Hospital Therapy and Immunology with a Postgraduate Course of the Professor Voino-Yasenetsky Krasnoyarsk State Medical University, endocrinologist of the Krasnoyarsk Regional Clinical Hospital
Competing Interests:
The author declares that he did not have any conflicts of interest in the planning, implementation, financing and use of the results of this study
Nadezhda N. Medvedeva
Professor Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk
Russian Federation
Competing Interests:
Russian Federation
Doctor of Medical Sciences, Professor, Head of the Department of Human Anatomy
Competing Interests:
The author declares that he did not have any conflicts of interest in the planning, implementation, financing and use of the results of this study
Andrey A. Savchenko
Krasnoyarsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk
Russian Federation
Competing Interests:
Russian Federation
Doctor of Medical Sciences, Professor, Head of the Laboratory of Molecular Cell Physiology and Pathology
Competing Interests:
The author declares that he did not have any conflicts of interest in the planning, implementation, financing and use of the results of this study
Anton V. Moshev
Krasnoyarsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk
Russian Federation
Competing Interests:
Russian Federation
Researcher of the Laboratory of Molecular Cell Physiology and Pathology
Competing Interests:
The author declares that he did not have any conflicts of interest in the planning, implementation, financing and use of the results of this study
Evgeny L. Zhukov
Professor Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk
Russian Federation
Competing Interests:
Russian Federation
Candidate of Medical Sciences, Associate Professor of the Professor Podzolkov Department of Pathological Anatomy
Competing Interests:
The author declares that he did not have any conflicts of interest in the planning, implementation, financing and use of the results of this study
Elena A. Hapilina
Professor Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk
Russian Federation
Competing Interests:
Russian Federation
Candidate of Medical Sciences, Associate Professor of the Department of Human Anatomy
Competing Interests:
The author declares that he did not have any conflicts of interest in the planning, implementation, financing and use of the results of this study
Gul'mira K. Kovalyova
Professor Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk
Russian Federation
Competing Interests:
Russian Federation
Candidate of Biological Sciences, Associate Professor of the Department of Histology, Cytology, Embryology
Competing Interests:
The author declares that he did not have any conflicts of interest in the planning, implementation, financing and use of the results of this study
Sergey A. Dogadin
Professor Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk Regional Clinical Hospital, Krasnoyarsk
Russian Federation
Competing Interests:
Russian Federation
Doctor of Medical Sciences, Professor of the Department of Hospital Therapy and Immunology with a course of postgraduate education of the Professor Voino-Yasenetsky Krasnoyarsk State Medical University, Head of the Endocrinology Centre of the Krasnoyarsk Regional Clinical Hospital
Competing Interests:
The author declares that he did not have any conflicts of interest in the planning, implementation, financing and use of the results of this study
Vladimir A. Man'kovsky
Krasnoyarsk Regional Clinical Hospital, Krasnoyarsk
Russian Federation
Competing Interests:
Russian Federation
Surgeon-Endocrinologist of the Krasnoyarsk Regional Clinical Hospital
Competing Interests:
The author declares that he did not have any conflicts of interest in the planning, implementation, financing and use of the results of this study
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Supplementary files
On the basis of a comprehensive clinical and morphological study, the authors showed the presence of parallels in changes in the immunological parameters of blood and thyroid tissue in patients with Graves' disease, which proves the role of cellular immune responses in the pathogenesis of the disease
Review
For citations:
Dudina M.A., Medvedeva N.N., Savchenko A.A., Moshev A.V., Zhukov E.L., Hapilina E.A., Kovalyova G.K., Dogadin S.A., Man'kovsky V.A. MORPHOLOGICAL FEATURES OF THYROID FOLLICLES AND THE CONSIST OF INTRATHYROID LYMPHOCYTES IN GRAVES' DISEASE. Morphological newsletter. 2021;29(2):15-26. (In Russ.)
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