THE OVARIECTOMIZED RATS BRAIN VENTRICLES STRUCTURAL COMPONENTS AFTER PROGESTERONE INJECTION STUDY

Progesterone and its neurosteroid derivatives play a fundamental role in ensuring trophic processes in hormone-dependent tissues, which in turn are of key importance in the development and functioning of the central nervous system. The concentration of progesterone in the brain is 20 times higher than in blood plasma. We did not find any literature data on the effect of progesterone on the morphology of the choroid plexus of the cerebral ventricles. In this regard, it is important to study the characteristics of the choroid plexus of the cerebral ventricles under the influence of progesterone. Objective: to study the morphological organization of the choroid plexus of the cerebral ventricles of ovariectomized Wistar rats under the influence of progesterone. The object of the morphological study was 20 female Wistar rats of sexually mature age. All animals were divided into two groups: 10 females in the control group and 10 females after ovariectomy with injection of progesterone at a dose of 250 mg per kg of animal weight. To assess the volume of the cerebral ventricles, sections were selected with their maximum cross-sectional area, the values ​​​​of the indicator were determined on digitized microphotographs at a magnification of 4 times by outlining the boundaries of the cerebral ventricles and subsequent calculations in the morphometric computer program ImageJ. Determination of volume fractions of vascular plexuses in the cerebral ventricles was carried out stereologically on serial sections using an ocular grid with 60 equally spaced intersection nodes at a magnification of 400 times. As a result of the study, it was revealed that progesterone has a significant effect on the structure of the vascular plexuses of all cerebral ventricles in ovariectomized animals. In the lateral ventricle, the maximum area increases, the relative volume of the vascular plexuses decreases, the volume fraction of the vessels inside the plexus increases, the volume fraction of the cells decreases, and the volume fraction of the connective tissue tends to increase. Similar dynamics are characteristic of the third and fourth ventricles.

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