In a study using histological and histochemical methods studied the amount of lymphatic and blood vessels in the prostate, pelvic structure and renal lymph nodes in experimental tumors in the prostate in CBA mice with Ehrlich’s ascites tumor transplantation at 5 and 18 days. On day 5, lymph node metastases were found, revealed characteristic morphological transformation: paracortical hyperplasia, sinus histiocytosis, activation of blast transformation. On day 18 in the prostate detected complete replacement of normal tissue with atypical cells in the lymph nodes are determined by metastases in the pelvic lymph nodes detected follicular response, increased transport function, against the backdrop o

The paper presents the study of the histostructure of suctorial pads extracted postmortem from neonates, children and adults of both sexes. The aim of the research is to the participation of suctoriak pads are organs which function throughout the entire postnatal period of human ontogenesis. Neonates and nursing infants have active intrascular erythro-and myelopoesis of suctoriak pads.

By means of use of morphological, histochemical, histoenzymatic methods there has been studied features of acetate gastric ulcer morphogenesis of 40 rats under chronic enteral hexachlorocyclohexane, chlorophos and copper sulphate injection. For the first time structural and metabolic basics of distortion of mucous membrane regeneration in ulcer area in continuous enteral toxic factor exposure and dose dependence of acute gastric ulcer chronicity are identified.

The adipose tissue content was determined by bioelectrical impedance analysis in mature women of different age groups with leptosomal, mesosomal and megalosomal constitution, somatotyped by method of I. Galant - B. Nikitjuk - V. Chtecov. The minimal absolute and relative value of the adipose content was registered in asthenical and stenoplastical somatotypes of all female age groups and women with picknical and euriplastical somatotypes had the maxmal numbers of this indicator. It was found the adipose body component increased in old age group. The study showed individual variability of adipose tissue content in all age female groups for every somatotype.

Bone marrow transplantation is widely used to treat cancer and diseases associated with the irradiated bone marrow. With luminescent-histochemical methods of research studied bioaminosoderzhaschie structure of the bone marrow, which include granular luminescent and mast cells involved in the regulation of immunogenesis. Objective: to study the effect of allograft bone marrow distribution in neyroaminov bioaminsoderzhaschih structures in the temporal aspect. We found that in 40 minutes after allogeneic bone marrow transplantation, decrease in the number of mast cells and granule, with decreasing granules therein, resulting in total decomposition. In these cells, reduced content neyroaminov. The myelogram sharply reduced the number of white blood cells mature forms. It is concluded that the allogenic bone marrow transplantation suppresses synthesis neyroaminov in bioaminosoderzhaschih cells.

Nanoscale materials are increasingly used to solve the fundamental problems and in applied biomedical research. The most well studied toxicity, biodistribution and biophysical properties of gold nanoparticles. They occupy a special place among the nanoscale materials used for diagnostic and therapeutic purposes, actively investigated as a nanosized tools for optical or magnetic imaging of cells and tissues, as carriers how of medicinal products for human and animal diagnostic preparations of the new generation. In the making of new drugs, the study of placental permeability barrier for them, as a rule, limited to the parameters of the general and selective toxicity study cellular responses mother and fetus, the nearest and distant teratogenicity. Nanotechnology can offer to solve the problem with more informative methods associated with drug conjugates. Nanopharmaceutics drugs can help in studies of mechanisms not only of placental permeability, but also of other blood-tissue barriers. Investigation of the mechanism of placental permeability barrier must be preceded by study of tissue and cellular responses of the placenta to the injection of nanotechnological therapeutic drugs. The purpose of this study is to investigate the morphological responses labyrinthine zone placenta white rat on parenteral administration of gold nanoparticles of different diameters. Gold nanoparticles with a diameter of 5, 10, 30, 50 and 150 nm, coated with polyethylene glycol was injected intravenously to pregnant female white rats at day 15 of gestation in the dose of 0.8 mg/kg of each animal. Gold nanoparticles in placental tissues were visualized by silver nitrate autometallography and total content of gold in the fetal tissues evaluated by atomic adsorption spectroscopy. With this method was studied the permeability of the rat’s placental barrier for gold nanoparticles with diameters of 5, 10, 30 and 50 nm in vivo. Morphological analysis of histological preparations of the placenta on the light optic level indicates the formation of non-specific adaptation reactions structures labyrinthine zone of the placenta, the expansion of blood vessels and capillaries, plethora, erythrocyte stasis, increasing of number of maternal blood lacunae. These findings suggest the need to consider the morphological responses of the labyrinthine zone placenta of white rats on parenteral injection of gold nanoparticles and their dimensional permeability to assess for the prospects of using new synthesized drugs with nanocarriers in veterinary and medical pharmaceutics.

The atomic force microscopy (hereinafter - AFM) is one of the leading applications of Nanotechnological research methods of cell membranes widely used in key areas of biomedicine. The study of the biophysical properties of the membrane and morphological parameters of lymphocytes by AFM can provide essential information about the patterns of structural change of the cytoplasmic membrane in the development of diabetes, disorders of metabolism, microcirculation and immunity. The purpose of this study was to investigate a number of morphological and biophysical characteristics of blood lymphocytes of patients with diabetes mellitus by AFM. The material of the study was lymphoid cells of peripheral blood of patients with insulin-dependent (IDDM) and non-insulin dependent type of diabetes mellitus (NIDDM), and healthy volunteers. For analysis of the membrane of lymphocytes was used a scanning probe microscope Solver P47-PRO (NT-MDT, Russia). Scanning cell surface of the membrane of blood lymphocytes were performed using silicon probes series PNP-DB-20 (NT-MDT) with a hardness of 0.06 N/m, with the radius of curvature of 10 nm. AFM images of the cells were analyzed using the computer program Nova. Based on analysis of AFM images were determined morphological parameters of blood lymphocytes: diameter, height, area, volume; and biophysical parameters: Young’s modulus, strength, adhesion, roughness. The results showed that in Diabetes mellitus is a significant increase in membrane rigidity blood lymphocytes as compared to those of the blood lymphocytes of healthy people. The adhesion properties and surface topography of lymphocytes in different types of diabetes is differing. At patients with IDDM values of adhesion strength and roughness of cells is higher than in patients with NIDDM. The morphometric parameters of blood lymphocytes of patients with IDDM also exceed similar parameters in patients with NIDDM. Identified using AFM morphological and biophysical characteristics of peripheral blood lymphocytes of patients with different types of diabetes may have a pathogenic role in the development of immunologic disorders occurring in this disease.

Histological dyes were obtained from oak bark and cherry branches and their staining properties were studied. It was shown that new dyes are cytoplasmic and can be used for staining of histological specimens of different organs. The dye based on the oak bark stains the cytoplasm of cells in dark brown or black color and does not stain their nuclei. The dye obtained from cherry branches gives moderately rich green color to the cytoplasm of cells.