A pilot study, which was performed on white laboratory male rats and included a morphological study of the mucous membrane of the jejunum and colon, revealed the long-term effect of a single oral exposure to an aqueous solution of depleted uranium oxides; this supported dynamic changes in the studied criteria determining metabolic, regenerative, and local regulatory processes in one, three and six months. A lymphoid component appeared to be the criterion of high radio sensitivity and a marker of nonspecific protection indicating deterioration of homeostasis. In the time dynamics of long-term periods, a change in all the parameters was detected when using immunohistochemical, histochemical, and specific methods at the level of the epithelium lining the relief formations of the mucous membranes of the intestinal system organs. The oxidative stress resulting in three months after exposure to depleted uranium, which was evidenced by a significant decrease in the content of anti-apoptotic Bcl-2 protein in the epithelial cells of the jejunely mucosa. Signs of its delayed effect after six months in the colon induced the pronounced nature of the reactions of stromal cell components that determined patterns of protective mechanisms of the intestinal-associated immune system with a greater expressiveness in the jejunum. The factual material cited in the work suggests the existence of a homeostatic immune response in the mucous membrane of the small and large intestines that controls cell proliferation, as a special form of immune surveillance of the state of cytodifferentiation.

In the epicardium of many mammals, animals, including humans, were found to have typical arteriovenular anastomoses. However, they do not correspond to the description of those direct anastomoses between arteries and veins located under the epicardium, which were previously identified. The authors who described them, using injection methods, showed the passage of carcass suspension from sub-epicardial arteries to sub-epicardial veins. But they could not accurately identify the type of vessels carrying out these connections. It is possible that these are arteriovenular anastomoses, since their diameter not exceeds the diameter of the blood capillaries. At the same time, it was shown that the vascular structures of the epicardium carry out the separation of the blood in the coronary artery system. As a result, blood with high hematocrit and freed from most various analytes and neutrophilic granulocytes enters the microvascular bed of the myocardium. The ultrafiltrate of the blood plasma in the sub-epicardial loose connective tissue undergoes biodegradation and is utilized in the lymphatic micro vessels. The aim of the work is the morphological and functional substantiation of the concept about of the epicardium as a biological filter of the heart. The vascular bed of the epicardium of hearts of mature dogs was studied (n=5), revealed by various routine histological and original intra- and extravascular methods. It is proved that the microvascular bloodstream of the sub-epicardial connective tissue has pronounced organ specificity. It is determined by the presence of arteriovenular anastomoses and permanent plasma capillaries, as well as the high permeability of all vascular components. The microvascular bed of the epicardium causes an ascending dilatation of the coronary arteries and contributes to ultrafiltration of a significant amount of blood plasma into the sub-epicardial interstitial space. Thus, the process of the separation of the blood is carried out, as a result of which blood with high hematocrit and free of neutrophilic granulocytes enters the myocardium. In the epicardial connective tissue, analytes accumulate that cannot be resorbed by venous micro vessels. This «biological debris» undergoes destruction in the sub-epicardial connective tissue, which proves the role of the epicardium as a biological filter of the heart.

The regeneration of the skin in the event of damage to the introduction of fillers is due to the resulting inflammatory reaction of the dermis. Among the vast group of fillers used in aesthetic medicine, polylactic acid, which is recommended as a “scaffold” forming factor that induces collagen synthesis by fibroblasts with subsequent transformation of this type of cells into myofibroblasts, has proven itself quite well. The aim of the study was to analyze the immunohistochemical status of the dermis with the introduction of an implant from polylactic acid. The study was performed on 30 rats with subdermal administration of the drug in a volume of 0.05 ml. Evaluation of the results was carried out two weeks later, 1, 2 and 4 months after the injection. Morphological evaluation was carried out in sections stained with hematoxylin and eosin according to Van Gieson and Mallory and Masson reactions. Immunohistochemical detection of type I and type III collagen was performed using polyclonal antibodies. Vimentin was used to assess the status of fibroblasts, and the marker CD68 to assess macrophages. The effect of remodeling of extracellular matrix was studied using α-SMA actin. It was shown that the response of the dermis to the implant is of a phase nature, begins with a subclinical inflammatory reaction, followed by encapsulation of each individual microsphere, and ends with dermal fibroplasia. This leads to the effect of increasing the volume of tissue and the result is achieved not only by properties of the introduced product, but also by the reaction of the body to its introduction. The detected activation of collagen synthesis by fibroblasts can be used as a regulator of the volume of the extracellular matrix of the dermis.

Successful reconstruction of the aortic valve requires the preliminary creation of the anatomical design of its image, which includes a set of parameters of the valves of a particular valve. Errors in operation at the stage of design of valve components are fraught with subsequent dysfunction. In the present, the optimal sizes of the aortic root and the valves of the aortic valve were evaluated and the presence and values of correlations between them were determined. Statistically significant anatomical and functional correlations of morphometric characteristics of aortic root structures were revealed to achieve the optimal level of their correspondence in autologous grafts during surgical correction of the aortic valve. The main morphometric parameters of the aortic root were analyzed on the example of 54 samples obtained post mortem from patients whose death occurred from causes not associated with heart disease. The cases with aortic valve disease were excluded from the study. The following parameters were evaluated: circumference of the ventriculo-arterial junction; circumference of the sinuses of Valsalva; circumference of the sinutubular junction. Separately, for each of the three cusps of the native aortic valve, were evaluated: the length of the free edge of the cusp (L1); the length of the junction of the leaflet and the fibrous ring of the aortic valve (L2); leaflet height (A); intercommissural distance (IC). A search was made for the correlation between the size of the valves and the parameters of the circumference of the aortic root at the level of the ventriculo-arterial junction, of the sinuses of Valsalva and of sinutubular junction. A significant presence of a correlations was found between all parameters of the aortic valve cusps and the length of the aortic circumference at the level of the ventriculo-arterial junction, of the sinuses of Valsalva and sinutubular junction (r>0.6 for IC, L1, L2 and r>0.5 for A at p<0,01). The data obtained by correlation analysis make it possible to standardize the design of the autograft of the aortic valve, thereby ensuring the best coaptation of the valves and, as a result, the function of the aortic valve.

Recent studies that analyze the cytotoxic and pro-carcinogenic effects of estrogens indicate an increased risk of ovarian cancer in those patients which are treatment and do not exclude the effect of excessive hormonal levels on the formation of morphological and functional changes in tissues and organs in future offspring. The aim of the study was to study the effects of the prenatal effects of subtoxic doses of synestrol on the general morphology, histometric and immunohistochemical parameters of the ovaries of the offspring of laboratory mice in the its postnatal reproductive period of ontogenesis. Pregnant female laboratory mice were divided into 3 groups. The control group of animals was once injected olive oil at a dose of 0,2 μg/kg intramuscularly. The first experimental group of animals was injected intramuscularly a single dose of synestrol at a dose of 25 μg/kg in the form of a 2% oil solution, the second experimental group was injected intramuscularly once a single subtoxic dose of synestrol of 40 μg/kg in the form of a 2% oil solution. All pregnant female mice were treatment drugs at the gestational stage of E11.5. A histological study of the preparations of the ovaries of the offspring of the first experimental group of mice showed a change in the ratio of the area of cortical substance relative to the area of the medullar substance with a decrease in the number of follicles at different stages of development compared with similar indicators of the offspring of the control group of animals. A single intramuscular injection of synestrol at a dose of 40 μg/kg in the offspring of the second experimental group led to an increase in the area of cortical and medullar substances of the ovaries, a decrease in the number of primordial, primary (unilaminar) follicles, in contrast to the ovaries of the control group. In histological sections of the ovaries of the second experimental group, hypertrophy and hyperplasia of granulosa cells, as well as teca interna follicles were observed. The appearance of atretic bodies, focal luteinization, indicating a real prenatal effect of the hormone on the postnatal structure of the organ, was noted in the cortical substance. A decrease in the activity of markers of cell proliferation, the transcription factor p53 of an anti-oncogene, indicated damage to ovarian functions at the level of their molecular genetic predictors. The results of the study generally showed that the effect of a subtoxic dose of synestrol at stage E11.5 of the prenatal period of development of the offspring of laboratory mice leads to persistent morphological changes in their ovaries in the postnatal reproductive period of ontogenesis.

The aging process of the skin of the hand continuously progresses from mature to advanced age. With age, the skin of the hand becomes pigmented, dry, wrinkled with an emphasized skin pattern, the tone of the skin of the hand decreases, thinning and atrophy of adipose tissue are noted, skin elasticity is lost with the formation of an excess of hypoelastic thinned dermis. In recent years, smooth thread implants have been used to correct involutional changes in the skin of the back of the hand. The aim of the study was a morphological evaluation of the effectiveness of the correction of involutional changes in the skin of the back surface of the hand with thread implants based on polycaprolactone and poly-L-lactic acid (PCL-PLLA) with notches. Using light and electron microscopy, we studied the reaction of cells and intercellular substance to the introduction of biodegradable filaments into the subcutaneous space. It was found that implantation of PCL-PLLA filaments increases the reparative potential of tissues. 3 months after implantation, stimulation of neocollagenesis and angiogenesis in the dermis and hypodermis was noted. The functional and proliferative activity of cells increased. Mitotic activity of cells in the basal layer of the epidermis led to an increase in its thickness. A clinically confirmed effect was noted after 3 months and persisted for 18 months after implantation. Long biodegradation of the threads (1.5-2 years) helps to slow down the aging process of the skin of the back surface of the hand, prolonging the processes of tissue revitalization.

The achievements of fundamental science have recently become the most widespread in the field of reproductive medicine. The biology of human development has mostly become understandable and manageable, thanks to new technologies. Some stages of the early human embryogenesis are predictably reproduced in the laboratories of assisted reproductive technologies (ART). Nevertheless, despite all the achievements, the success (birth of a healthy child) does not exceed 30% of the started cycles. That is why there is a continual search for new methods and their combinations to achieve better results and develop standard protocols for managing patients with infertility. The purpose of this work is to analyze the feasibility of genetic screening of embryos (NGS) and to compare the effectiveness of using donor and own oocytes when transferring a single embryo in cryocycles. We have analyzed the 536 cryocycles. There were four groups of patients with single embryo transfer (SET): group 1 - cycles with NGS, embryos with genetically euploid status taken for selection for transfer with used own oocytes (NSd, n=20); group 2 - cycles with single embryo transfer without NGS and using own oocytes (nSd, n=446); group 3 - cycles without NGS, using donor oocytes (nSD, n=8). All groups 1, 2, and 3 were near the same with an average age (34,1 - 34,3 - 34,6 years, respectively). Due to the small size of group 3, another (4th) observation group was taken (cycles without NGS, using donor oocytes, nSD, n=62), but without age restrictions, where the average age of patients was 42.3 years. Analyze of the survival rate shows significant decreasing in the group used donor cells (3 groups) than in groups (1 and 2) used own cells (84.62% vs 100%, p=0.060 and 91.96%, p<0.0001, respectively). The age difference in the groups with donor cells (3 and 4) did not significantly affect the survival rate (84.82% and 92.94%, p=0.443). We found differences in the rate of HCG(+) between groups using donor oocytes (groups 3 and 4) and own oocytes without genetic screening (group 2), where the indicator was significantly lower (62.5% and 62.98% vs 41.03%, p<0.0001 in both cases). The same significant differences in the pregnancy rate (PR) and implantation rate (IR) we found between group 2 (own oocytes without screening) and all other groups (1, 3 and 4). In the last groups, the indicators were significantly higher (PR - 35.2% against 60%, 62.5% and 58.06%, respectively, and IR - 36.36% against 60%, 62.5% and 56.45%, respectively). The results we obtained confirm the definitive role of oocyte competencies, demonstrate the absence of an adverse effect of vitrification on embryos after trophoblast biopsy, and convincingly prove the feasibility and significant positive impact of genetic screening of embryos on clinical results.

There are a number of publications in the literature that describe osteometric measurements of the styloid process of the temporal bone. Most studies are based on the results of direct measurements of bone preparations of the skulls; however, modern radiation research allows you to get a complete picture of the shape, relative position with other anatomical structures. Obtained results are rare. The purpose of the study was to present the results of measurements of the styloid process of the temporal bone and its relationships with other parts of the stylo-hyoidal complex obtained by analyzing images of multi-spiral computed tomography in patients without pathology of this anatomical zone. The length and width of the process, the distance to the small horn of the hyoid bone, and the height and weight of patients depending on age were measured. It has been established that multi-spiral computed tomography is an accurate method for determining the parameters of the styloid process. No statistically significant relationships between the anatomical parameters of the styloid process, other elements of the stylo-hyoidal complex, gender, height and age of the examined patients were found.

The problem of variant anatomy of blood vessels does not lose its relevance, it comprehensively is being studied still and important in modern human anatomy. The study of the diversity of branching of the arterial bed is of interest not only to anatomists, but also to practicing physicians. The anatomical and clinical Russian and English-language medical literature describes numerous variants of the structure of the arterial system regarding the place of branching, the presence of additional arteries and the absence of major arteries, their branching types. During the preparation of corpses intended for practical studies with students at the Department of Human Anatomy, anatomists are often found with variability of branching vessels. Based on many years of cadavers’ preparation experience, it can be said that almost every corpse has certain structural features of the vascular system that are different from the classical description. Variants and anomalies of vascular branching can affect the pathogenesis and symptoms of various diseases, as well as the choice of methods for their treatment. When dissecting a female corpse, atypical branching of the axillary and brachial arteries was discovered. The aim of the study was to describe the revealed vascular anomaly of this corpse. In the study of the upper third of the shoulder was found a high fission of a. brachialis on its two main branches - a. ulnaris and a. radialis. A. thoracica lateralis was absent. Moreover, a. thoracoacromialis was branched from a. axillaris in the area of the thoracic triangle. This case is an example of atypical branching of the brachial and axillary arteries. Knowledge of branching options for arteries is necessary for carrying out diagnostic and treatment manipulations, since such atypical branching types undoubtedly complicate the work during surgical procedures during surgical interventions in this area of the limb, which, in fact, can lead to iatrogenic damage of arteries.

Currently, the problem of echinococcosis remains relevant, since this chronically ongoing and parasitic disease leading to disability occurs in different age groups. In this study, the goal was to conduct a morphological analysis of the fibrous capsule with adjacent liver tissues, depending on the type of echinococcal cysts. The analysis of histological preparations of postoperative biopsies of the liver of 32 patients with echinococcosis after surgery on the liver . A standard morphological analysis of the preparations of the sites of the chitinous membrane and liver parenchyma stained with hematoxylin-eosin and Van Gieson was performed. Types of echinococcal cysts were classified according to the Apoyan-Sarkisyan (1991) classification according to ultrasound sonography and compared with types of cysts according to the international classification of ultrasound images for cystic echinococcosis, which was approved by the WHO. It was found that fibrous membranes in cysts of type II (CE1), III (CE2) and IV (CE3) types may contain germ elements, therefore, this types must be considered as one of the factors for the occurrence of recurrence of echinococcosis.

The need to organize a medical institute in the center of Soviet Russia was caused by difficult socio-economic conditions, an increase in the incidence of the population, and insufficient provision of medical care in the 30s of the 20th century. It was rational to found the new medical university in the city of Kursk to provide of the doctors training with the western part of the Central Black Earth Region. The decision of the Soviet Russia Government was implemented in 1935. One of the first to form and begin work was the Department of Normal Anatomy. The material base for ensuring the educational process at this difficult time was collected bit by bit and required a long time. However, the Great Patriotic War soon ensued and the department as part of the institute was evacuated to Alma-Ata in 1941. After the evacuation in 1943 its employees had to restore both the premises and the provision of the educational process with cadavers. The first heads of the department L.A. Shangina, M.K. Mavromati, N.V. Dobroserdov in the 40-50s began the formation of the anatomical museum and laid the foundations for scientific activity, which were mainly devoted to the study of the lymphatic system. From 1954 to 1966 under the heading of Professor A.A. Otelin were made significant changes to the educational process, the results of more than 40 morphological studies were published and 4 dissertations were defended. The main significant transformations occurred at the department during the years of Professor D.A. Sigalevich heading, 23 candidates and 4 doctoral dissertations were defended, a complete reconstruction of the anatomical museum was made, and many methodological manuals were created. Followers of Professor D.A. Sigalevich - E.M. Smolyar, E.P. Borzilov, N.N. Kaznacheev were keepers and enhanced the good traditions and staff of the department. Since 1999, the head of the department is Professor V.V. Kharchenko, who completely changed the face of the department, equipping it with new technical teaching aids, modern environmentally friendly preparations.